The study of the prophylactic effectiveness of “kalcifostilis”, an orally administrated preparation in preventing hypocalcemia and hypophosphatemia in dairy cow

Establishing the change dynamics of certain macroelements, glucose and parathormone in the blood of a pregnant cow. Developing a preparation to be used as a preventive measure for metabolic disorders in cows, and establishing its effectiveness based on the results of a biochemical and clinical study. Goals: 1. Establishing the change dynamics of calcium, inorganic phosphorus, magnesium, glucose and parathormone in the blood of a cow during pregnancy, during calving and postpartum. 2. Establishing the change in the amounts of macroelements (Ca, Pn, Mg) in the blood serum of a cow after oral administration of Kalcifostilis. 3. Examining therapeutic and prophylactic effectiveness of Kalcifostilis to prevent hypocalcaemia and hypophosphatemia in cows. 4. Establishing prophylactic effectiveness of Kalcifostilis in cows that previously suffered from parturition paresis, brought on by clinical hypocalcaemia and hypophosphatemia. Kalcifostilis, an orally administered preparation for the prevention of mineral metabolism disorders in cows, was developed by the Experimental and Clinical Pharmacology Laboratory of the Lithuanian Veterinary Academy. We researched the change in the amounts of calcium, inorganic phosphorus, magnesium, glucose and parathormone in the blood serum of a cow during pregnancy, during calving and postpartum. We also established those change parameters in the blood serum of a cow after the insorption of the component parts of the preparation. The obtained data allowed us to pinpoint the most critical period of time for developing clinical and subclinical mineral metabolism diseases, with relation to calving time. We established the optimum time to administer Kalcifostilis and developed its usage pattern. Kalcifostilis is manufactured by UAB Ruvera and used in practice. Conclusion: 1. Establishing the change in the amount of calcium, inorganic phosphorus and parathormone in the blood of a cow at the end of the pregnancy, during calving and after calving, allows to diagnose hypocalcaemia and hypophosphatemia at an early stage, as well as choose the most optimal time for administering Kalcifostilis. 2. The macroelements’ change during pregnancy shows that the most critical time for developing hypocalcaemia and hypophosphatemia is the first day after calving, when the amount of calcium and inorganic phosphorus falls (up to 1.2±0.6 mmol/l and 0.76±0.43 mmol/l respectively) below the physiological standard. 3. There is a decrease in the amount of calcium in the blood of a cow on the day of calving, at the same time there is an increase in the amount of parathormone (from 1.15±0.35 to 9.12±4.39 µmol/l), which encourages the absorption of the aforementioned mineral from the bone. 4. After administering Kalcifostilis right before calving, as well as once or twice after, the amount of calcium and inorganic phosphorus in cows’ blood, compared to that of the cows which weren’t given the preparation, significantly increased (p